Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation, progressive heterochromatinization, and DNA fragmentation

被引：151

作者：

Gernand, D ^{[1
]}

Rutten, T ^{[1
]}

Varshney, A ^{[1
]}

Rubtsova, M ^{[1
]}

Prodanovic, S ^{[1
]}

Brüss, C ^{[1
]}

Kumlehn, J ^{[1
]}

Matzk, F ^{[1
]}

Houben, A ^{[1
]}

机构：

[1] Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany

来源：

PLANT CELL | 2005年 / 17卷 / 09期

关键词：

D O I：

10.1105/tpc.105.034249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Complete uniparental chromosome elimination occurs in several interspecific hybrids of plants. We studied the mechanisms underlying selective elimination of the paternal chromosomes during the development of wheat ( Triticum aestivum) 3 pearl millet ( Pennisetum glaucum) hybrid embryos. All pearl millet chromosomes were eliminated in a random sequence between 6 and 23 d after pollination. Parental genomes were spatially separated within the hybrid nucleus, and pearl millet chromatin destined for elimination occupied peripheral interphase positions. Structural reorganization of the paternal chromosomes occurred, and mitotic behavior differed between the parental chromosomes. We provide evidence for a novel chromosome elimination pathway that involves the formation of nuclear extrusions during interphase in addition to postmitotically formed micronuclei. The chromatin structure of nuclei and micronuclei is different, and heterochromatinization and DNA fragmentation of micronucleated pearl millet chromatin is the final step during haploidization.

引用

页码：2431 / 2438

页数：8

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