Expression of the rabbit cytochrome P450 aromatase encoding gene uses alternative tissue-specific promoters

被引：16

作者：

Bouraïma, H ^{[1
]}

Hanoux, V ^{[1
]}

Mittre, H ^{[1
]}

Féral, C ^{[1
]}

Benhaïm, A ^{[1
]}

Leymarie, P ^{[1
]}

机构：

[1] Univ Caen, CHU Cote Nacre, USC INRA, UPRES EA 2608,Lab Biochim, F-14032 Caen, France

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 16期

关键词：

rabbit; P450; aromatase; alternative promoters; granulosa cells; corpus luteum;

D O I：

10.1046/j.1432-1327.2001.02375.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to analyse the tissue-specific expression of various promoter-derived transcripts from the gene encoding rabbit aromatase cytochrome P450. A new promoter, named I.r, was identified, and promoters II and I.r were sequenced. Promoter I.r-derived transcripts were found in preovulatory granulosa cells, corpus luteum, placenta and adipose tissue. An alternative splice variant of this transcript was found with tissue-specific preference. Tissue-specific expression of promoter-derived variants was studied in the ovary before and after ovulation. While the level of promoter II-derived transcript decreased dramatically after ovulation, that of promoter I.r-derived transcript remained unchanged, indicating that promoter II and promoter I.r were not controlled by a single regulation system. The existence of this dual system of regulation suggests that the rabbit ovary could be a useful model to study the promoter-specific regulation of aromatase.

引用

页码：4506 / 4512

页数：7

共 32 条

[1] Luteotrophic factors in hyperstimulated pseudopregnant rabbit .1. Evidence for aromatase activity in luteal tissue and luteal cells [J].

Arioua, RK ;

Feral, C ;

Benhaim, A ;

Delarue, B ;

Leymarie, P .

JOURNAL OF ENDOCRINOLOGY, 1997, 154 (02) :249-257

[2] Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts equine granulosa cells during human chorionic gonadotropin-induced ovulation: A novel model for the study of aromatase promoter switching [J].

Boerboom, D ;

Kerban, A ;

Sirois, J .

ENDOCRINOLOGY, 1999, 140 (09) :4133-4141

[3] Functional interactions, phosphorylation, and levels of 3',5'-cyclic adenosine monophosphate-regulatory element binding protein and steroidogenic factor-1 mediate hormone-regulated and constitutive expression of aromatase in gonadal cells [J].

Carlone, DL ;

Richards, JS .

MOLECULAR ENDOCRINOLOGY, 1997, 11 (03) :292-304

[4] Closely related genes encode developmental and tissue isoforms of porcine cytochrome P450 aromatase [J].

Choi, IH ;

Troyer, DL ;

Cornwell, DL ;

KirbyDobbels, KR ;

Collante, WR ;

Simmen, FA .

DNA AND CELL BIOLOGY, 1997, 16 (06) :769-777

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6]

Delarue B, 1996, CR ACAD SCI III-VIE, V319, P663

[7] Expression of two aromatase cDNAs in various rabbit tissues [J].

Delarue, B ;

Breard, E ;

Mittre, H ;

Leymarie, P .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1998, 64 (1-2) :113-119

[8]

FERAL C, 1990, J REPROD FERTIL, V89, P407, DOI 10.1530/jrf.0.0890407

[9]

FROHMAN MA, 1993, METHOD ENZYMOL, V218, P340

[10] Tissue-specific expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative first exons [J].

Furbass, R ;

Kalbe, C ;

Vanselow, J .

ENDOCRINOLOGY, 1997, 138 (07) :2813-2819

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