Introns and their positions affect the translational activity of mRNA in plant cells

被引:81
作者
Bourdon, V [1 ]
Harvey, A [1 ]
Lonsdale, DM [1 ]
机构
[1] John Innes Ctr, Norwich NR4 7UH, Norfolk, England
关键词
D O I
10.1093/embo-reports/kve090
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In an attempt to further increase transgene expression levels in plants over and above the enhancement obtained with a 5' untranslated leader intron, three different maize introns were inserted at three different positions within the coding sequence of the luciferase reporter gene. Constructs were transformed into maize (Black Mexican Sweet) cells and protoplasts, and their activity determined. Although all introns tested were correctly spliced, only one of them in a particular position was able to enhance gene expression. Correct splicing sites were used for intron removal and the quantity of luciferase mRNA produced did not differ significantly. These data indicate that both the position and the sequence of an intron have marked effects on expression levels, suggesting that nuclear processing of the pre-mRNA determines final expression levels through the structure of the mRNP.
引用
收藏
页码:394 / 398
页数:5
相关论文
共 21 条
[1]   A CATALOG OF SPLICE JUNCTION AND PUTATIVE BRANCH POINT SEQUENCES FROM PLANT INTRONS [J].
BROWN, JWS .
NUCLEIC ACIDS RESEARCH, 1986, 14 (24) :9549-9559
[2]   COMPARISON OF INTRON-DEPENDENT AND INTRON-INDEPENDENT GENE-EXPRESSION [J].
BUCHMAN, AR ;
BERG, P .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) :4395-4405
[3]   INTRONS INCREASE GENE-EXPRESSION IN CULTURED MAIZE CELLS [J].
CALLIS, J ;
FROMM, M ;
WALBOT, V .
GENES & DEVELOPMENT, 1987, 1 (10) :1183-1200
[4]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[5]   INTRONS IN THE 3'-UNTRANSLATED REGION CAN INHIBIT CHIMERIC CAT AND BETA-GALACTOSIDASE GENE-EXPRESSION [J].
EVANS, MJ ;
SCARPULLA, RC .
GENE, 1989, 84 (01) :135-142
[6]  
FENNELL A, 1992, PLANT CELL REP, V11, P567, DOI 10.1007/BF00233094
[7]  
FROMM M, 1987, METHOD ENZYMOL, V153, P351
[8]   THE REGULATION OF GENE-EXPRESSION IN TRANSFORMED MAIZE ALEURONE AND ENDOSPERM PROTOPLASTS - ANALYSIS OF PROMOTER ACTIVITY, INTRON ENHANCEMENT, AND MESSENGER-RNA UNTRANSLATED REGIONS ON EXPRESSION [J].
GALLIE, DR ;
YOUNG, TE .
PLANT PHYSIOLOGY, 1994, 106 (03) :929-939
[9]   Isolation and characterization of the wheat prolyl isomerase FK506-binding protein (FKBP) 73 promoter [J].
Kurek, I ;
Harvey, AJ ;
Lonsdale, DM ;
Breiman, A .
PLANT MOLECULAR BIOLOGY, 2000, 42 (03) :489-497
[10]  
Le Hir H, 2000, GENE DEV, V14, P1098