The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite

被引：196

作者：

Ogata, H ^{[1
]}

Renesto, P

Audic, S

Robert, C

Blanc, G

Fournier, PE

Parinello, H

Claverie, JM

Raoult, D

机构：

[1] CNRS, IBSM, UPR 2589, Struct & Genom Informat Lab, Marseille, France

[2] Fac Med Marseille, CNRS, IFR 48, UMR 6020,Unite Rickettsies, F-13385 Marseille, France

来源：

PLOS BIOLOGY | 2005年 / 3卷 / 08期

关键词：

D O I：

10.1371/journal.pbio.0030248

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular alpha-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short ( 39,263 bp) and a long ( 62,829 bp) form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin - antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as beta-lactamase activity, actin-polymerization-driven mobility, and hemolytic properties. Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria.

引用

页码：1391 / 1402

页数：12

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