Linear SRY transcript in equine testis

被引：16

作者：

Hasegawa, T ^{[1
]}

Ishida, M

Harigaya, T

Sato, F

Ishida, N

Mukoyama, H

机构：

[1] Japan Racing Assoc, Equine Res Inst, Mol & Cellular Biol Lab, Utsunomiya, Tochigi 3200856, Japan

[2] Meiji Univ, Fac Agr, Funct Anat Lab, Kawasaki, Kanagawa 2140033, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1999年 / 61卷 / 01期

关键词：

equine; sex-determination; SRY;

D O I：

10.1292/jvms.61.97

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Employing a combination of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques, the complete coding sequence of cDNA for the equine SRY gene was determined. We also attempted to make clear whether the equine SRY gene transcript is expressed in the adult testis, and whether the type of transcript is expressed as linear or circular RNA. As a result, in total a 1420 bp cDNA sequence was determined. Accomplishment of 3' RACE infers that equine SRY gene was expressed as a linear RNA transcript in testicular tissue just after puberty, in contrast to the situation in mice.

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页码：97 / 100

页数：4

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