Expression of prokaryotic 1-deoxy-D-xylulose-5-phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis

被引:118
作者
Harker, M [1 ]
Bramley, PM [1 ]
机构
[1] Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Egham TW20 0EX, Surrey, England
来源
FEBS LETTERS | 1999年 / 448卷 / 01期
关键词
isoprenoid; isopentenyl diphosphate; carotenoid; bioengineering; ubiquinone; biosynthesis;
D O I
10.1016/S0014-5793(99)00360-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Isopentenyl diphosphate (IPP) acts as the common, five-carbon building block in the biosynthesis of all isoprenoids. The first reaction of IPP biosynthesis in Escherichia coli is the formation of 1deoxy-D-xylulose-5-phosphate, catalysed by 1-deoxy-D-xylulose-5-phosphate synthase (DXPS). E. coli engineered to produce lycopene, was transformed with dxps genes cloned from Bacillus subtilis and Synechocystis sp. 6803. Increases in lycopene levels were observed in strains expressing exogenous DXPS compared to controls. The recombinant strains also exhibited elevated levels of ubiquinone-8. These increases corresponded with enhanced DXP synthase activity in the recombinant E. coli strains. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:115 / 119
页数:5
相关论文
共 34 条
[1]  
[Anonymous], 1996, Dictionary of Natural Products
[2]   Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits [J].
Bouvier, F ;
d'Harlingue, A ;
Suire, C ;
Backhaus, RA ;
Camara, B .
PLANT PHYSIOLOGY, 1998, 117 (04) :1423-1431
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   ISOLATION AND STRUCTURAL CHARACTERIZATION OF A CDNA-ENCODING ARABIDOPSIS-THALIANA 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE [J].
CAELLES, C ;
FERRER, A ;
BALCELLS, L ;
HEGARDT, FG ;
BORONAT, A .
PLANT MOLECULAR BIOLOGY, 1989, 13 (06) :627-638
[5]   DISTRIBUTION OF ISOPRENOID QUINONE STRUCTURAL TYPES IN BACTERIA AND THEIR TAXONOMIC IMPLICATIONS [J].
COLLINS, MD ;
JONES, D .
MICROBIOLOGICAL REVIEWS, 1981, 45 (02) :316-354
[6]  
Collins MD., 1985, METHOD MICROBIOL, P329, DOI DOI 10.1016/S0580-9517(08)70480-X
[7]   Monoterpenoid essential oils are not of Mevalonoid origin [J].
Eisenreich, W ;
Sagner, S ;
Zenk, MH ;
Bacher, A .
TETRAHEDRON LETTERS, 1997, 38 (22) :3889-3892
[8]   Studies on the biosynthesis of taxol: The taxane carbon skeleton is not of mevalonoid origin [J].
Eisenreich, W ;
Menhard, B ;
Hylands, PJ ;
Zenk, MH ;
Bacher, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6431-6436
[9]   A COMMON STRUCTURAL MOTIF IN THIAMIN PYROPHOSPHATE-BINDING ENZYMES [J].
HAWKINS, CF ;
BORGES, A ;
PERHAM, RN .
FEBS LETTERS, 1989, 255 (01) :77-82
[10]  
Hemmi H, 1998, J BIOCHEM-TOKYO, V123, P1088