Expression of prokaryotic 1-deoxy-D-xylulose-5-phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis

被引：118

作者：

Harker, M ^{[1
]}

Bramley, PM ^{[1
]}

机构：

[1] Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Egham TW20 0EX, Surrey, England

来源：

FEBS LETTERS | 1999年 / 448卷 / 01期

关键词：

isoprenoid; isopentenyl diphosphate; carotenoid; bioengineering; ubiquinone; biosynthesis;

D O I：

10.1016/S0014-5793(99)00360-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isopentenyl diphosphate (IPP) acts as the common, five-carbon building block in the biosynthesis of all isoprenoids. The first reaction of IPP biosynthesis in Escherichia coli is the formation of 1deoxy-D-xylulose-5-phosphate, catalysed by 1-deoxy-D-xylulose-5-phosphate synthase (DXPS). E. coli engineered to produce lycopene, was transformed with dxps genes cloned from Bacillus subtilis and Synechocystis sp. 6803. Increases in lycopene levels were observed in strains expressing exogenous DXPS compared to controls. The recombinant strains also exhibited elevated levels of ubiquinone-8. These increases corresponded with enhanced DXP synthase activity in the recombinant E. coli strains. (C) 1999 Federation of European Biochemical Societies.

引用

页码：115 / 119

页数：5

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