X11L2, a new member of the X11 protein family, interacts with Alzheimer's β-amyloid precursor protein

被引：73

作者：

Tanahashi, H ^{[1
]}

Tabira, T ^{[1
]}

机构：

[1] Natl Inst Neurosci, Div Demyelinating Dis & Aging, Tokyo 1878502, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.0265

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We screened proteins for interaction with Alzheimer's beta-amyloid precursor protein (APP) and cloned a new member of the X11 protein family, X11L2. The PID/PTB element of X11L2 protein interacted with the intracellular domain of APP by GST binding assay, and in vivo interaction was confirmed by coimmunoprecipitation from cell extracts overexpressing APP and HA-tagged X11L2. This gene encoded 575 amino acids and the deduced amino acid sequence was highly homologous to rat Mint3. Three protein-protein interaction domains, a PID/PTB and two PDZ elements, were conserved among the X11 protein family, and the N-terminal region of X11L2 protein had several putative SH3 binding motifs, PXXP. Unlike other members of the X11 protein family, X11L2 mRNA was expressed in various tissues. (C) 1999 Academic Press.

引用

页码：663 / 667

页数：5

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