Quenching of biotinylated aequorin bioluminescence by dye-labeled avidin conjugates: Application to homogeneous bioluminescence resonance energy transfer assays

被引：18

作者：

Adamczyk, M ^{[1
]}

Moore, JA ^{[1
]}

Shreder, K ^{[1
]}

机构：

[1] Abbott Labs, Dept Chem, Div Diagnost, Abbott Pk, IL 60064 USA

来源：

ORGANIC LETTERS | 2001年 / 3卷 / 12期

关键词：

D O I：

10.1021/ol015843p

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] Avidin conjugates containing the covalently attached dyes QSY-7 and dabcyl were prepared and shown to quench the bioluminescence of biotinylated aequorin. Quenching efficiency was shown to be dependent on both the label to avidin ratio and the concentration of the avidin conjugate, These properties were exploited to develop a homogeneous bioluminescence resonance energy transfer (BRET) assay for biotin.

引用

页码：1797 / 1800

页数：4

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