Chemotaxis receptor complexes: From signaling to assembly

被引:14
作者
Endres, Robert G. [1 ]
Falke, Joseph J.
Wingreen, Ned S.
机构
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[2] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
关键词
D O I
10.1371/journal.pcbi.0030150
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Complexes of chemoreceptors in the bacterial cytoplasmic membrane allow for the sensing of ligands with remarkable sensitivity. Despite the excellent characterization of the chemotaxis signaling network, very little is known about what controls receptor complex size. Here we use in vitro signaling data to model the distribution of complex sizes. In particular, we model Tar receptors in membranes as an ensemble of different sized oligomer complexes, i.e., receptor dimers, dimers of dimers, and trimers of dimers, where the relative free energies, including receptor modification, ligand binding, and interaction with the kinase CheA determine the size distribution. Our model compares favorably with a variety of signaling data, including dose-response curves of receptor activity and the dependence of activity on receptor density in the membrane. We propose that the kinetics of complex assembly can be measured in vitro from the temporal response to a perturbation of the complex free energies, e. g., by addition of ligand.
引用
收藏
页码:1385 / 1393
页数:9
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