Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

被引：730

作者：

Draper, JS

Smith, K

Gokhale, P

Moore, HD

Maltby, E

Johnson, J

Meisner, L

Zwaka, TP

Thomson, JA

Andrews, PW

机构：

[1] Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England

[2] Sheffield Childrens Trust, N Trent Clin Cytogenet Serv, Sheffield S10 2TH, S Yorkshire, England

[3] Univ Sheffield, Royal Hallamshire Hosp, Sect Reprod & Dev Med, Sheffield S10 2SF, S Yorkshire, England

[4] Wisconsin State Lab Hyg, Cytogenet Lab, Madison, WI 53706 USA

[5] Univ Wisconsin, Natl Primate Res Ctr, Sch Med, Madison, WI 53715 USA

[6] Univ Wisconsin, Dept Anat, Sch Med, Madison, WI 53715 USA

来源：

NATURE BIOTECHNOLOGY | 2004年 / 22卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1038/nbt922

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

引用

页码：53 / 54

页数：2

共 15 条

[1] Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture [J].