Application of a UV-vis detection-HPLC method for a rapid determination of lycopene and β-carotene in vegetables

The purpose of this paper is to optimize an HPLC method for the determination of lycopene and beta-carotene in vegetables and compare it with a spectrophotometric standard method. Among the different conditions studied the most suitable ones for our samples were: extraction with hexane/acetone/ethanol (50:25:25 v/v/v), evaporation of the hexane layer, dissolution of the dry extract in THF/ACN/methanol (15:30:55 v/v/v) and injection on a C18 column with methanol/ACN (90:10 v/v) + TEA 9 mu M as mobile phase (Phi = 0.9 ml/min) and lambda(detection) = 475 nm. Samples considered for analysis were: tomato, carrot, pepper, watermelon, persimmon and medlar. The HPLC method proposed showed adequate reproducibility (RSD < 10.5%), accuracy (100-109% recovery) and sensitive detection limits (0.6 mu M for lycopene; 0.3 mu M for beta-carotene), with a simple preparation of the samples (one step direct extraction) and short run times (10 min) for the quantification of lycopene and beta-carotene. (c) 2005 Elsevier Ltd. All rights reserved.