Detection of Echinococcus multilocularis in foxes in the Netherlands

被引:73
作者
van der Giessen, JWB [1 ]
Rombout, YB [1 ]
Franchimont, JH [1 ]
Limper, LP [1 ]
Homan, WL [1 ]
机构
[1] Natl Inst Publ Hlth & Environm, Microbiol Lab Hlth Protect, NL-3720 BA Bilthoven, Netherlands
关键词
Echinococcus multilocularis; fox; PCR; epidemiology; Nematoda; The Netherlands;
D O I
10.1016/S0304-4017(98)00263-5
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Echinococcus multilocularis was demonstrated in 5 out of 272 foxes in The Netherlands close to the border with Germany and Belgium. Besides microscopic examination of mucosal scrapings, two different PCR assays were used based on the detection of E. multilocularis DNA in colon content. Two distinct areas in The Netherlands were positive for E. multilocularis. Two positive foxes were found in the northern province of Groningen and three positive foxes were found in the southern province of Limbug. Both PCR assays detected more positive foxes compared to microscopic examination of the intestinal content. This is the first report of E. multilocularis in foxes occurring in The Netherlands. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:49 / 57
页数:9
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