Transfer of recombinant human granulocyte colony stimulating factor (rhG-CSF) from the maternal to the fetal circulation is not dependent upon a functional G-CSF-receptor

被引:8
作者
Calhoun, DA [1 ]
Gersting, JA [1 ]
Lunoe, M [1 ]
Du, Y [1 ]
Christensen, RD [1 ]
机构
[1] Univ Florida, Coll Med, Dept Pediat, Div Neonatol, Gainesville, FL 32610 USA
关键词
D O I
10.1053/plac.2001.0682
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Administration of granulocyte colony stimulating factor (G-CSF), a haematopoietic growth factor, to pregnant rats increases neutrophil production in the pugs. The mechanism for the placental transfer is unknown, but it has been speculated to involve the placental G-CSF receptor (G-CSFR). The purpose of this study was to test that hypothesis. Pregnant mice were treated with a single subcutaneous dose of 50 mug/kg recombinant human G-CSF (rhG-CSF). Mice with an intact G-CSFR ('wild type',WT) and those with a homozygous deletion in the G-CSFR gene (G-CSFR deficient, 'knock-out', KO) were studied. At intervals after injection, fetuses were delivered and maternal blood, amniotic fluid (AF) and fetal blood collected. G-CSF concentrations were measured using an enzyme linked immunosorbent assay specific for human G-CSF. Thirty minutes after injection, G-CSF was measurable in the AF (167 +/- 50 versus 445 +/- 217 pg/ml, mean +/- SEM, WT versus KO) and fetal plasma (774 +/- 673 versus 427 +/- 121 pg/ml, WT versus KO). Peak concentrations occurred 2 h after injection in WT dams (572 542 +/- 41 262 pg/ml) and 4 h in KO dams (616 100 +/- 96 300 pg/ml). Therefore, in mice, a functional G-CSFR is not essential for the transfer of rhG-CSF from pregnant dams to their fetuses. (C) 2001 Harcourt Publishers Ltd.
引用
收藏
页码:609 / 612
页数:4
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