Preparative asymmetric reduction of ketones in a biphasic medium with an (S)-alcohol dehydrogenase under in situ-cofactor-recycling with a formate dehydrogenase

被引：80

作者：

Gröger, H

Hummel, W

Rollmann, C

Chamouleau, F

Hüsken, H

Werner, H

Wunderlich, C

Abokitse, K

Drauz, K

Buchholz, S

机构：

[1] Degussa AG, Project House Biotechnol, D-63403 Hanau, Germany

[2] Univ Dusseldorf, Forschungszentrum, Inst Mol Enzymtechnol, D-52426 Julich, Germany

[3] Degussa AG, SU Process Technol, D-63403 Hanau, Germany

[4] Degussa AG, BU Fine Chem, FC TRM, D-63450 Hanau, Germany

来源：

TETRAHEDRON | 2004年 / 60卷 / 03期

关键词：

ketones; in situ-cofactor regeneration; (S)-alcohol;

D O I：

10.1016/j.tet.2003.11.066

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The substrate range of a novel recombinant (S)-alcohol dehydrogenase from Rhodococcus erythropolis is described. In addition, an enzyme-compatible biphasic reaction medium for the asymmetric biocatalytic reduction of ketones with in situ-cofactor regeneration has been developed. Thus, reductions of poorly water soluble ketones in the presence of the alcohol dehydrogenase from R. erythropolis and a formate dehydrogenase from Candida boidinii can be carried out at higher substrate concentrations of 10-200 mM. The resulting (S)-alcohols were formed with moderate to good conversion rates, and with up to >99% ce. (C) 2003 Published by Elsevier Ltd.

引用

页码：633 / 640

页数：8

共 37 条

[1] Cloning, sequence analysis, and heterologous expression of the gene encoding a (S)-specific alcohol dehydrogenase from Rhodococcus erythropolis DSM 43297 [J].