Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis

被引：12

作者：

Zhang, XF ^{[1
]}

Ma, Y ^{[1
]}

Liu, H ^{[1
]}

de Sa, PFG ^{[1
]}

Brown, PR ^{[1
]}

Dain, JA ^{[1
]}

机构：

[1] Univ Rhode Isl, Dept Chem, Kingston, RI 02881 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 325卷 / 02期

关键词：

MRP; AGE; glucosamine; fibrinogen; human serum albumin; capillary electrophoresis;

D O I：

10.1016/j.ab.2003.11.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：255 / 259

页数：5

共 16 条

[1]

de Sa Paula F G, 2002, J Capill Electrophor Microchip Technol, V7, P61

[2] The use of capillary electrophoresis to monitor Maillard reaction products (MRP) by glyceraldehyde and the epsilon amino group of lysine [J].