Sensitive determination of bisphenol A and alkylphenols by high performance liquid chromatography with precolumn derivatization with 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole

被引:8
作者
Katayama, M
Sasaki, T
Matsuda, Y
Kaneko, S
Iwamoto, T
Tanaka, M
机构
[1] Meiji Coll Pharm, Dept Funct Bioanal, Tokyo 2048588, Japan
[2] Tokyo Dent Coll, Ichikawa Gen Hosp, Dept Obstet & Gynecol, Chiba 2720824, Japan
[3] St Marianna Univ, Sch Med, Dept Urol, Kawasaki, Kanagawa 2688511, Japan
[4] St Marianna Univ, Sch Med, Dept Pharmacol, Kawasaki, Kanagawa 2688511, Japan
关键词
D O I
10.1002/bmc.88.abs
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new and sensitive high-performance chromatographic method for the determination of bisphenol A and 8 alkylphenols with fluorescence detection is reported. F-ach phenol was derivatized by reaction with 2-(4-carboxyphenyl)-5,6dimethylbenzimidazole at 40 degreesC for 60 min. The fluorescence derivatives were separated on a Wakosil 5C18 column (4.0 i.d. x 300 mm, 5 mum) with methanol:water (10:90) as mobile phase (detection wavelength: lambda (ex) 336 nm, lambda (em) 440 nm). The detection limits were in the range of 0.1-10.0 pg/mL in serum. The calibration graphs were linear to 1.0 mug/mL. The relative standard deviations were 7.2-8.9%, respectively. The proposed method was applied to the determination of bisphenol A in mother and infant rat serum. Copyright (C) 2001 John Wiley & Sons, Ltd.
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页码:403 / 407
页数:5
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