HYDROMIC: prediction of hydrodynamic properties of rigid macromolecular structures obtained from electron microscopy images

被引：42

作者：

de la Torre, JG ^{[1
]}

Llorca, O

Carrascosa, JL

Valpuesta, JM

机构：

[1] Univ Murcia, Dept Quim Fis, E-30071 Murcia, Spain

[2] CSIC, Ctr Nacl Biotecnol, Madrid, Spain

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2001年 / 30卷 / 06期

关键词：

HYDROMIC; hydrodynamic coefficients; solution properties; macromolecules; electron microscopy;

D O I：

10.1007/s002490100176

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have developed a procedure for the prediction of hydrodynamic coefficients and other solution properties of macromolecules and macromolecular complexes whose volumes have been generated from electron microscopy images. Starting from the structural files generated in the three-dimensional reconstructions of such molecules, it is possible to construct a hydrodynamic model for which the solution properties can be calculated. We have written a computer program, HYDROMIC, that implements all the stages of the calculation. The use of this procedure is illustrated with a calculation of the solution properties of the volume of the cytosolic chaperonin CCT, obtained from cryoelectron microscopy images.

引用

页码：457 / 462

页数：6

共 27 条

[1] FRICTIONAL COEFFICIENTS OF MULTISUBUNIT STRUCTURES .1. THEORY [J].