Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS: identification of ceramides containing sphingadienine

被引：53

作者：

Colsch, B

Afonso, C

Popa, L

Portoukalian, J

Fournier, F

Tabet, JC

Baumann, N ^{[1
]}

机构：

[1] Hop La Pitie Salpetriere, Lab Neurochim, INSERM, U495, F-75651 Paris 13, France

[2] Univ Paris 06, CNRS, UMR 7613, F-75252 Paris 05, France

[3] INSERM, U346, F-75654 Paris 13, France

[4] Hop Edouard Herriot, Dermatol Lab, F-69003 Lyon, France

来源：

JOURNAL OF LIPID RESEARCH | 2004年 / 45卷 / 02期

关键词：

ion trap; triple quadrupole; precursor ion scan; electrospray ionization tandem mass spectrometry;

D O I：

10.1194/jlr.M300331-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases. Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.

引用

页码：281 / 286

页数：6

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