Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

被引：14

作者：

Gutjahr, C

Murphy, D

Lueking, A

Koenig, A

Janitz, M

O'Brien, J

Korn, B

Horn, S

Lehrach, H

Cahill, DJ

机构：

[1] Royal Coll Surgeons Ireland, Ctr Human Prote, Dublin 2, Ireland

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

[3] Ruhr Univ Bochum, Med Proteome Ctr, D-44780 Bochum, Germany

[4] Royal Coll Surgeons Ireland, Dept Clin Pharmacol, Dublin 2, Ireland

[5] Resource Ctr Genome Res, D-69120 Heidelberg, Germany

[6] PROTAGEN AG, D-44227 Dortmund, Germany

来源：

GENOMICS | 2005年 / 85卷 / 03期

基金：

爱尔兰科学基金会;

关键词：

T(H)1; cDNA expression library; automation; mouse; high-density protein arrays; microarrays; protein chips; serum profiling; SLE; autoantibody;

D O I：

10.1016/j.ygeno.2004.11.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T-H 1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse T-H 1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：285 / 296

页数：12

共 46 条

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