A monoclonal antibody that inhibits Trypanosoma cruzi growth in vitro and its reaction with intracellular triosephosphate isomerase

被引：21

作者：

Cortes-Figueroa, A. A. ^{[1
,2
]}

Perez-Torres, A. ^{[3
]}

Salaiza, N. ^{[1
]}

Cabrera, N. ^{[4
]}

Escalona-Montano, A. ^{[1
]}

Rondan, A. ^{[3
]}

Aguirre-Garcia, M. ^{[1
]}

Gomez-Puyou, A. ^{[4
]}

Perez-Montfort, R. ^{[4
]}

Becker, I. ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Expt Med, Fac Med, Mexico City 06726, DF, Mexico

[2] Ctr Nacl Noviembre 20, Mexico City, DF, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Biol Celular & Tisular, Mexico City 06726, DF, Mexico

[4] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 06726, DF, Mexico

来源：

PARASITOLOGY RESEARCH | 2008年 / 102卷 / 04期

关键词：

D O I：

10.1007/s00436-007-0803-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学]; 100103 [病原生物学];

摘要：

In parasites of the order Kinetoplastida, such as Trypanosoma cruzi and Trypanosoma brucei, glycolysis is carried out by glycolytic enzymes in glycosomes. One of the glycolytic enzymes is triosephosphate isomerase (TIM), which in T. brucei is localized exclusively in glycosomes, whereas in T. cruzi, the localization of TIM has not been fully ascertained. In the present work, we made a monoclonal antibody (mAb 6-11G) against recombinant T. cruzi TIM (rTcTIM). Incubation of T. cruzi epimastigotes with the mAb inhibited parasite survival. Western blotting showed that the mAb recognized rTcTIM and a 27 kDa band in T. cruzi lysates that corresponded to TcTIM. Sera from patients with Chagas disease recognized rTcTIM and cross-reacted with human recombinant TIM. The cross reactivity between parasite and human TIM possibly contributes to the autoimmune pathogenesis of Chagas disease. Electron microscopy of T. cruzi epimastigotes with the mAb showed that TIM was located within glycosomes, in the cytoplasm, the nucleus, and the kinetoplast. Collectively, the data shed new light on T. cruzi TIM and opens perspectives for drug design.

引用

页码：635 / 643

页数：9

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