Immobilization of α-amylase in vinyl-polymer-based interpenetrating polymer networks

被引:43
作者
Bajpai, AK [1 ]
Bhanu, S [1 ]
机构
[1] Govt Autonomous Sci Coll, Dept Chem, Bose Mem Res Lab, Jabalpur 482001, MP, India
关键词
interpenetrating polymer network; alpha-amylase; immobilization; kinetics;
D O I
10.1007/s00396-003-0921-2
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Semi-interpenetrating polymer networks (IPNs) of poly(ethylene glycol), poly(vinyl alcohol) and polyacrylamide were prepared as a support for enzyme immobilization and kinetic studies were performed for the immobilization of alpha-amylase. The effect of IPN composition on the extent of immobilization was investigated and the percentage of relative activity of the immobilized enzyme was evaluated as a function of the chemical architecture of the IPNs, pH and temperature, taking starch as a substrate. The kinetic constants and the maximum reaction velocity were also evaluated. The IPNs were characterized by IR spectral analysis.
引用
收藏
页码:76 / 83
页数:8
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