A new matrix for membrane affinity chromatography and its application to the purification of concanavalin A

被引：51

作者：

Guo, W ^{[1
]}

Ruckenstein, E ^{[1
]}

机构：

[1] SUNY Buffalo, Dept Chem Engn, Buffalo, NY 14260 USA

来源：

JOURNAL OF MEMBRANE SCIENCE | 2001年 / 182卷 / 1-2期

关键词：

affinity membranes; chromatography; crosslinked filter paper membrane; concanavalin A purification;

D O I：

10.1016/S0376-7388(00)00574-3

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Macroporous cellulose membranes with large pore sizes (1-2 mum) and high porosity (about 60%) were prepared from filter paper by chemical crosslinking. These membranes were activated by introducing epoxy groups with various spacer lengths. after which maltose was immobilized as an affinity ligand. packed into a cartridge, the prepared membranes were used for the affinity purification of concanavalin A. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：227 / 234

页数：8

共 23 条

[1] Separation of paraproteins from human plasma by membrane chromatography [J].