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A microarray strategy for mapping the substrate specificity of protein tyrosine phosphatase

被引：57
作者：
Koehn, Maja
Gutierrez-Rodriguez, Marta
Jonkheijm, Pascal
Wetzel, Stefan
Wacker, Ron
Schroeder, Hendrik
Prinz, Heino
Niemeyer, Christof M.
Breinbauer, Rolf
Szedlacsek, Stefan E.
Waldmann, Herbert
机构：
[1] Max Planck Inst Mol Physiol, Dept Biol Chem, D-44277 Dortmund, Germany
[2] Univ Dortmund, Fac Chem, D-44277 Dortmund, Germany
[3] Chimera Biotec GmbH, D-44227 Dortmund, Germany
[4] Univ Dortmund, Fac Chem Biol Chem Mikrostrkturtech, D-44277 Dortmund, Germany
[5] Inst Biochem, Dept Enymol, Bucharest 060031, Romania
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 40期
关键词：
enzymes; inhibitors; microarrays; peptides; substrate specificity;
D O I：
10.1002/anie.200701601
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Which substrate will it be? Phosphotyrosine peptide microarrays have allowed the substrate specificity to be mapped for two prototypical protein-tyrosine phosphatases (PTPs): PTP1B and PTPμ. The knowledge gained was used for molecular docking studies (see picture: the docking of a peptide in the binding pocket of PTPμ) and the design of an inhibitor for PTPμ. (Figure Presented). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.
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收藏
页码：7700 / 7703
页数：4
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