Peptide-sugar ligation catalyzed by transpeptidase sortase: A facile approach to neoglycoconjugate synthesis

被引:88
作者
Samantaray, Sharmishtha [1 ]
Marathe, Uttara [1 ]
Dasgupta, Sayani [1 ]
Nandicoori, Vinay K. [1 ]
Roy, Rajendra P. [1 ]
机构
[1] Natl Inst Immunol, New Delhi 110067, India
关键词
D O I
10.1021/ja077358g
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Glycoconjugate synthesis involving sugar and polypeptide remains a formidable challenge. Here we report a novel enzymatic method involving an unprecedented sortase-catalyzed transamidation reaction for site-specific engineering of sugars into native proteins. We show that sugars appended with a 6-aminohexose moiety can be efficiently ligated to peptides; and proteins encoded with a LPXTG sortase, recognition sequence. This robust reaction provides an elegant and simple approach for generating neoglycoproteins with an amide-linked sugar moiety at the carboxy terminus.
引用
收藏
页码:2132 / +
页数:3
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