Carbapenem-Hydrolyzing GES-Type Extended-Spectrum β-Lactamase in Acinetobacter baumannii

被引：95

作者：

Bonnin, Remy A. ^{[1
,2
]}

Nordmann, Patrice ^{[1
,2
]}

Potron, Anais ^{[1
,2
,3
]}

Lecuyer, Herve ^{[3
]}

Zahar, Jean-Ralph ^{[3
]}

Poirel, Laurent ^{[1
,2
]}

机构：

[1] Hop Bicetre, AP HP, Fac Med, INSERM,U914,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

[2] Univ Paris Sud, F-94275 Le Kremlin Bicetre, France

[3] Hop Necker Enfants Malad, AP HP, Lab Microbiol Hyg Hosp, Paris, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 01期

关键词：

RESISTANT PSEUDOMONAS-AERUGINOSA; BIOCHEMICAL-CHARACTERIZATION; MULTIDRUG-RESISTANCE; INFECTIONS; MECHANISMS; DIVERSITY; VARIANT; DNA;

D O I：

10.1128/AAC.00773-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Acinetobacter baumannii isolate AP was recovered from a bronchial lavage of a patient hospitalized in Paris, France. A. baumannii AP was resistant to all beta-lactams, including carbapenems, and produced the extended-spectrum beta-lactamase (ESBL) GES-14, which differs from GES-1 by two substitutions, Gly170Ser and Gly243Ala. Cloning of the bla(GES-14) gene followed by its expression in Escherichia coli showed that GES-14 compromised significantly the efficacy of all beta-lactams, including cephalosporins, aztreonam, and carbapenems. The carbapenemase activity of purified GES-14 was confirmed by kinetic studies. The bla(GES-14) gene was located into a class 1 integron structure and located onto a ca. 95-kb self-transferable plasmid. This study identified a very broad-spectrum beta-lactamase in A. baumannii.

引用

收藏

页码：349 / 354

页数：6

相关论文

共 30 条

[1] A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES [J].

AMBLER, RP ;

COULSON, AFW ;

FRERE, JM ;

GHUYSEN, JM ;

JORIS, B ;

FORSMAN, M ;

LEVESQUE, RC ;

TIRABY, G ;

WALEY, SG .

BIOCHEMICAL JOURNAL, 1991, 276 :269-270

[2]

[Anonymous], CLIN MICROBIOL REV

[3] Genetic and biochemical characterization of GES-5, an extended-spectrum class A β-lactamase from Klebsiella pneumoniae [J].

Bae, Il Kwon ;

Lee, You-Nae ;

Jeong, Seok Hoon ;

Hong, Seong Geun ;

Lee, Jung Hun ;

Lee, Sang Hee ;

Kim, Hyoung Jin ;

Youn, Hasik .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 58 (04) :465-468

[4] Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa [J].

Bonomo, Robert A. ;

Szabo, Dora .

CLINICAL INFECTIOUS DISEASES, 2006, 43 :S49-S56

[5]

Clinical and Laboratory Standards Institute, 2010, CLSI M100S20

[6] Bacterial identification, clinical significance, and antimicrobial susceptibilities of Acinetobacter ursingii and Acinetobacter schindleri, two frequently misidentified opportunistic pathogens [J].

Dortet, Laurent ;

Legrand, Patrick ;

Soussy, Claude-James ;

Cattoir, Vincent .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (12) :4471-4478

[7] Comparative genomics of multidrug resistance in Acinetobacter baumannii [J].

Fournier, Pierre-Edouard ;

Vallenet, David ;

Barbe, Valerie ;

Audic, Stephane ;

Ogata, Hiroyuki ;

Poirel, Laurent ;

Richet, Herve ;

Robert, Catherine ;

Mangenot, Sophie ;

Abergel, Chantal ;

Nordmann, Patrice ;

Weissenbach, Jean ;

Raoult, Didier ;

Claverie, Jean-Michel .

PLOS GENETICS, 2006, 2 (01) :62-72

[8] IBC-1, a novel integron-associated class A β-lactamase with extended-spectrum properties produced by an Enterobacter cloacae clinical strain [J].

Giakkoupi, P ;

Tzouvelekis, LS ;

Tsakris, A ;

Loukova, V ;

Sofianou, D ;

Tzelepi, E .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (09) :2247-2253

[9] OXA-143, a Novel Carbapenem-Hydrolyzing Class D β-Lactamase in Acinetobacter baumannii [J].

Higgins, Paul G. ;

Poirel, Laurent ;

Lehmann, Marlene ;

Nordmann, Patrice ;

Seifert, Harald .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (12) :5035-5038

[10] Inverse Correlation between Promoter Strength and Excision Activity in Class 1 Integrons [J].

Jove, Thomas ;

Da Re, Sandra ;

Denis, Francois ;

Mazel, Didier ;

Ploy, Marie-Cecile .

PLOS GENETICS, 2010, 6 (01)