A real-time fluorescence polymerase chain reaction assay for the identification of Yersinia pestis using a field-deployable thermocycler

被引：10

作者：

McAvin, JC ^{[1
]}

McConathy, MA

Rohrer, AJ

Huff, WB

Barnes, WJ

Lohman, KL

机构：

[1] USAF, Inst Environm Safety & Occupat Hlth Risk Anal, Mol Epidemiol Branch, Epidemiol Surveillance Div, Brooks AFB, TX 78235 USA

[2] USAF, IERA, Brooks AFB, TX 78235 USA

[3] USAF Acad, Colorado Springs, CO 80840 USA

来源：

MILITARY MEDICINE | 2003年 / 168卷 / 10期

关键词：

D O I：

10.1093/milmed/168.10.852

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Real-time fluorescence polymerase chain reaction is a microbial identification method that can provide rapid and accurate results using a field-deployable thermocycler, the RAPID ("ruggedized" advanced pathogen identification device). A Yersinia pestis-specific TaqMan assay required approximately 75 minutes and achieved a sensitivity of 100 fg of Y. pestis genomic DNA (20 genome equivalents). Specificity testing against a genomic DNA cross-reaction panel comprised of 22 bacterial species encountered in the respiratory tract resulted in no false positives. No c ross-reaction occurred with human genomic DNA.

引用

页码：852 / 855

页数：4

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