Spermatogenesis-specific association of SMCY and MSH5

被引：47

作者：

Akimoto, Chihiro ^{[1
]}

Kitagawa, Hirochika ^{[1
]}

Matsumoto, Takahiro ^{[1
,2
]}

Kato, Shigeaki ^{[1
,2
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

[2] Japan Sci & Technol Agcy, ERATO, Kawaguchi, Saitama 3320012, Japan

来源：

GENES TO CELLS | 2008年 / 13卷 / 06期

关键词：

D O I：

10.1111/j.1365-2443.2008.01193.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The status of chromatin during spermatogenesis is dynamically regulated by specific histone codes or stage-specific histone changes. The functional links between such epigenetic regulation and proteins regulating meiosis are largely unknown. In mammals, genes encoded on the Y chromosome are thought to possess male-specific biological functions. While genes located within the azoospermia factor region (AZF) are known to be involved in spermatogenesis, the physiological function of individual genes is not known. SMCY is a gene mapped to the AZF, and in this report, we analyzed the function of SMCY protein during spermatogenesis. Biochemical identification of the proteins with which it interacted showed that SMCY formed a distinct complex with MSH5, a critical meiosis-regulatory protein in the human testicular germ cell line, NEC8. As anticipated, histone H3K4 demethylase activity was detected. Immunohistochemical analysis revealed the co-localization of SMCY with MSH5 at a specific stage of meiotic prophase progression during murine spermatogenesis. Our results suggest that SMCY may have a male-specific function as a histone H3K4 demethylase by recruiting a meiosis-regulatory protein to condensed DNA.

引用

页码：623 / 633

页数：11

共 40 条

[1] Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis [J].

Börner, GV ;

Kleckner, N ;

Hunter, N .

CELL, 2004, 117 (01) :29-45

[2] Mouse MutS-like protein Msh5 is required for proper chromosome synapsis in male and female meiosis [J].

de Vries, SS ;

Baart, EB ;

Dekker, M ;

Siezen, A ;

de Rooij, DG ;

de Boer, P ;

te Riele, H .

GENES & DEVELOPMENT, 1999, 13 (05) :523-531

[3] Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility [J].

Eddy, EM ;

Washburn, TF ;

Bunch, DO ;

Goulding, EH ;

Gladen, BC ;

Lubahn, DB ;

Korach, KS .

ENDOCRINOLOGY, 1996, 137 (11) :4796-4805

[4] Mammalian MutS homologue 5 is required for chromosome pairing in meiosis [J].

Edelmann, W ;

Cohen, PE ;

Kneitz, B ;

Winand, N ;

Lia, M ;

Heyer, J ;

Kolodner, R ;

Pollard, JW ;

Kucherlapati, R .

NATURE GENETICS, 1999, 21 (01) :123-127

[5] Meiotic pachytene arrest in MLH1-deficient mice [J].

Edelmann, W ;

Cohen, PE ;

Kane, M ;

Lau, K ;

Morrow, B ;

Bennett, S ;

Umar, A ;

Kunkel, T ;

Cattoretti, G ;

Chaganti, R ;

Pollard, JW ;

Kolodner, RD ;

Kucherlapati, R .

CELL, 1996, 85 (07) :1125-1134

[6] RETRACTED: Ligand-induced transrepression by VDR through association of WSTF with acetylated histones (retracted article) [J].

Fujiki, R ;

Kim, MS ;

Sasaki, Y ;

Yoshimura, K ;

Kitagawa, H ;

Kato, S .

EMBO JOURNAL, 2005, 24 (22) :3881-3894

[7] RETRACTED: DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs (Retracted article. See vol. 16, pg. 1126, 2014) [J].

Fukuda, Toru ;

Yamagata, Kaoru ;

Fujiyama, Sally ;

Matsumoto, Takahiro ;

Koshida, Iori ;

Yoshimura, Kimihiro ;

Mihara, Masatomo ;

Naitou, Masanori ;

Endoh, Hideki ;

Nakamura, Takashi ;

Akimoto, Chihiro ;

Yamamoto, Yoko ;

Katagiri, Takenobu ;

Foulds, Charles ;

Takezawa, Shinichiro ;

Kitagawa, Hirochika ;

Takeyama, Ken-ichi ;

O'Malley, Bert W. ;

Kato, Shigeaki .

NATURE CELL BIOLOGY, 2007, 9 (05) :604-U221

[8] The XY body: a specialized meiotic chromatin domain [J].

Handel, MA .

EXPERIMENTAL CELL RESEARCH, 2004, 296 (01) :57-63

[9] A histone H3 methyltransferase controls epigenetic events required for meiotic prophase [J].

Hayashi, K ;

Yoshida, K ;

Matsui, Y .

NATURE, 2005, 438 (7066) :374-378

[10] Meisetz, a novel histone tri-methyltransferase, regulates meiosis-specific epigenesis [J].

Hayashi, Katsuhiko ;

Matsui, Yasuhisa .

CELL CYCLE, 2006, 5 (06) :615-620

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