The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves

被引：127

作者：

Bozkurt, Ahmet ^{[1
]}

Lassner, Franz ^{[1
]}

O'Dey, Dan ^{[1
]}

Deumens, Ronald ^{[2
,3
]}

Boecker, Arne ^{[1
]}

Schwendt, Tilman ^{[4
]}

Janzen, Christoph ^{[5
]}

Suschek, Christoph V. ^{[1
]}

Tolba, Rene ^{[6
]}

Kobayashi, Eiji ^{[7
]}

Sellhaus, Bernd ^{[2
]}

Tholl, S. ^{[8
]}

Eummelen, Lizette ^{[8
]}

Schuegner, Frank ^{[8
]}

Damink, Leon Olde ^{[8
]}

Weis, Joachim ^{[2
]}

Brook, Gary A. ^{[2
]}

Pallua, Norbert ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Fac Med, Dept Plast Surg Reconstruct & Hand Surg, Burn Ctr, D-52074 Aachen, Germany

[2] Rhein Westfal TH Aachen, Fac Med, Univ Hosp, Inst Neuropathol, D-52074 Aachen, Germany

[3] Maastricht Univ, Med Ctr, Dept Anesthesiol, Maastricht, Netherlands

[4] Rhein Westfal TH Aachen, Fraunhofer Inst Laser Technol, Aachen, Germany

[5] Rhein Westfal TH Aachen, Chair Laser Technol, Aachen, Germany

[6] Rhein Westfal TH Aachen, Fac Med, Inst Lab Anim Res, D-52074 Aachen, Germany

[7] Jichi Med Univ, Ctr Dev Adv Med Technol, Shimotsuke, Tochigi, Japan

[8] Matricel GmbH, Herzogenrath, Germany

来源：

BIOMATERIALS | 2012年 / 33卷 / 05期

关键词：

Nerve regeneration; Schwann cells; Scaffold; Neurotmesis; Sciatic nerve; Bands of Bungner; FUNCTIONAL RECOVERY; SCHWANN-CELLS; IN-VITRO; SPINAL-CORD; TISSUE; SCAFFOLDS; SYSTEM; MATRIX; REPAIR; RAT;

D O I：

10.1016/j.biomaterials.2011.10.069

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1363 / 1375

页数：13

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