Ultrasonic-trap-enhanced selectivity in capillary electrophoresis

被引:40
作者
Wiklund, M [1 ]
Spégel, P
Nilsson, S
Hertz, HM
机构
[1] Royal Inst Technol, SE-10691 Stockholm, Sweden
[2] Ctr Chem & Chem Engn, SE-22100 Lund, Sweden
关键词
ultrasonic trapping; particle separation; capillary electrophoresis; concentration limit of detection;
D O I
10.1016/S0041-624X(02)00460-2
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-muM diam capillary of the CE system. Competition between acoustic and viscous forces result in inflow separation of mum-diam spheres according to their size. Experiments separating 2.8- and 2.1-mum-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 10(4) compared to current CE systems. Theoretical calculations show room for further improvement. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:329 / 333
页数:5
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