Quantitative electron spectroscopic imaging In bio-medicine: Evaluation and application

被引:11
作者
Beckers, ALD
Gelsema, ES
DeBruijn, WC
CletonSoeteman, MI
VanEijk, HG
机构
[1] ERASMUS UNIV ROTTERDAM, INST PATHOL, AEM UNIT, NL-3000 DR ROTTERDAM, NETHERLANDS
[2] ERASMUS UNIV ROTTERDAM, DEPT CHEM PATHOL, NL-3000 DR ROTTERDAM, NETHERLANDS
关键词
biochemistry; EELS; electron energy-loss spectroscopy; electron spectroscopic imaging; ESI; ferritin; haemochromatosis; haemosiderin; image processing; iron;
D O I
10.1046/j.1365-2818.1996.72437.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
Electron spectroscopic imaging (ESI) with the energy-filtering transmission electron microscope enables the investigation of chemical elements in ultrathin biological sections. An analysis technique has been developed to calculate elemental maps and quantitative distributions from ESI sequences. Extensive experience has been obtained with a practical implementation of this technique. A procedure for more robust element detection has been investigated and optimized. With the use of Fe-loaded Chelex beads, the measurement system has been evaluated with respect to the linearity of the element concentration scale, the reproductibility of the measurements and the visual usage of images results. In liver specimens of a patient with an iron storage disease the detectability of iron was tested and we tried to characterize iron-contained components. The concentration measurement scale is approximately linear up to a relative section thickness of approximate to 0.5. Monitoring of this parameter is therefore considered to be important. The reproducibility was measured in an experiment with Fe-Chelex. The iron concentration differed by 6.4% between two serial measurements. Element distributions are in many applications interpreted visually. For this purpose the frequently used net-intensity distributions are regarded as unsuitable. For the quantification and visual interpretation of concentration differences mass thickness correction has to be performed. By contrast, for the detection of elements the signal-to-noise ratio is the appropriate criterion. Application of ESI analysis demonstrated the quantitative chemical capabilities of this technique in the investigation of iron storage diseases. Based on an assumed ferritin iron loading in vivo, different iron components can be discerned in liver parenchymal cells of an iron-overloaded patient.
引用
收藏
页码:78 / 88
页数:11
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