Single ion channel sensitivity in suspended bilayers on micromachined supports

被引：56

作者：

Cheng, YL ^{[1
]}

Bushby, RJ ^{[1
]}

Evans, SD ^{[1
]}

Knowles, PF ^{[1
]}

Miles, RE ^{[1
]}

Ogier, SD ^{[1
]}

机构：

[1] Univ Leeds, Ctr Self Organising Mol Syst, Leeds LS2 9JT, W Yorkshire, England

来源：

LANGMUIR | 2001年 / 17卷 / 04期

关键词：

D O I：

10.1021/la001423h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper describes the formation of bilayer lipid membranes on a micromachined support, consisting of a 128 mum (diameter) aperture on a gold/SU8 surface suspended over a small aqueous reservoir (similar to 25 nL). The bilayers are highly impermeable to ion transport with specific resistance values up to 10(7) Omega cm(2) Single-channel activities of gramicidin and alamethicin were observed in these systems. This work demonstrates that micromachined supports for bilayers are promising for the development of a biosensor with single-channel sensitivity.

引用

页码：1240 / 1242

页数：3

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