Arcobacter cibarius sp nov., isolated from broiler carcasses

被引:117
作者
Houf, K
On, SLW
Coenye, T
Mast, J
Van Hoof, J
Vandamme, P
机构
[1] State Univ Ghent, Fac Med Vet, Dept Vet Publ Hlth & Food Safety, B-9820 Merelbeke, Belgium
[2] Danish Vet Inst, DK-1790 Copenhagen, Denmark
[3] State Univ Ghent, Fac Sci, Microbiol Lab, B-9000 Ghent, Belgium
[4] Vet & Agrochem Res Ctr, B-1180 Brussels, Belgium
关键词
D O I
10.1099/ijs.0.63103-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Twenty Gram-negative, rod-shaped, slightly curved, non-spore-forming bacteria that gave a negative result in Arcobacter species-specific PCR tests but that yielded an amplicon in an Arcobacter genus-specific PCR test were isolated from 13 unrelated broiler carcasses. Numerical analysis of the profiles obtained by SDS-PAGE of whole-cell proteins clustered all isolates in a single group distinct from the other Arcobacter species. DNA-DNA hybridization among four representative strains exhibited DNA binding values above 91%. DNA-DNA hybridization with reference strains of the current four Arcobacter species revealed binding levels below 47%. The G + C contents ranged between 26.8 and 27.3 mol%. Pairwise comparison of 16S rRNA gene sequences revealed the mean values for similarity to the type strain of Arcobacter cryaerophilus (97.5%), Arcobacter butzleri (96.5%), Arcobacter skirrowii (96.0%) and Arcobacter nitrofigilis (95.0%). The levels of similarity to Campylobacter and Helicobacter species were below 88 and 87%, respectively. The isolates could be distinguished from other Arcobacter species by the following biochemical tests: catalase, oxidase and urease activities; reduction of nitrate; growth at 25 and 37 degrees C under aerobic conditions; growth on 2-4% (w/v) NaCl media; and susceptibility to cephalothin. These data demonstrate that the 20 isolates represent a single novel Arcobacter species, for which the name Arcobacter cibarius sp. nov. is proposed, with LMG 21996(T) (=CCUG 48482(T)) as the type strain.
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页码:713 / 717
页数:5
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