Acquisition of different carbapenem resistance mechanisms by an epidemic clonal lineage of Pseudomonas aeruginosa

被引：25

作者：

Edalucci, E. ^{[1
]}

Spinelli, R. ^{[1
]}

Dolzani, L. ^{[1
]}

Riccio, M. Letizia ^{[2
]}

Dubois, V. ^{[3
]}

Tonin, E. Angelo ^{[1
]}

Rossolini, G. M. ^{[2
]}

Lagatolla, C. ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Biomed, Sez Microbiol, I-34127 Trieste, Italy

[2] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy

[3] Univ Bordeaux 2, CNRS, UMR 5234, Microbiol Lab, F-33076 Bordeaux, France

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2008年 / 14卷 / 01期

关键词：

carbapenem resistance; clonal lineage; metallo-beta-lactamase; Pseudomonas aeruginosa; resistance acquisition; VIM-2;

D O I：

10.1111/j.1469-0691.2007.01874.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Multidrug-resistant isolates of a clonal lineage of Pseudomonas aeruginosa producing the VIM-2 metallo-beta-lactamase (MBL), involved in a large outbreak in an Italian hospital, were compared with MBL-negative strains that had caused outbreaks in two French hospitals. Although the isolates had different carbapenem MICs, the VIM-2-producing isolates from Italy carried identical, or very similar, allelic forms of the oprD gene, harboured a common class 1 integron, belonged to the same multilocus sequence type (ST111), and showed macrorestriction profiles that were related to those of the MBL-negative French strains. These results support the concept of independent acquisition of resistance determinants by members of a widespread clonal lineage of P. aeruginosa.

引用

页码：88 / 90

页数：3

共 19 条

[1] Genomic typing of Pseudomonas aeruginosa isolates by comparison of Riboprinting and PFGE:: correlation of experimental results with those predicted from the complete genome sequence of isolate PAO1 [J].

Botes, J ;

Williamson, G ;

Sinickas, V ;

Gürtler, V .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 55 (01) :231-240

[2]

Bush K, 2001, CLIN INFECT DIS, V32, P1085, DOI 10.1086/319610

[3] In99, an In100-related integron, its occurrence and prevalence in clinical Pseudomonas aeruginosa strains from a central region of Portugal [J].

Caetano, T. ;

Ferreira, S. ;

Mondego, A. P. ;

Correia, A. ;

Mendo, S. .

EPIDEMIOLOGY AND INFECTION, 2007, 135 (03) :502-504

[4]

*CLIN LAB STAND I, 2006, M100S16 CLSI

[5] Development of a multilocus sequence typing scheme for the opportunistic pathogen Pseudomonas aeruginosa [J].

Curran, B ;

Jonas, D ;

Grundmann, H ;

Pitt, T ;

Dowson, CG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (12) :5644-5649

[6] Nosocomial outbreak due to a multiresistant strain of Pseudomonas aeruginosa P12:: Efficacy of cefepime-amikacin therapy and analysis of β-lactam resistance [J].

Dubois, V ;

Arpin, C ;

Melon, M ;

Melon, B ;

Andre, C ;

Frigo, CC ;

Quentin, C .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) :2072-2078

[7] Raising antibodies against OprD, an outer membrane protein of Pseudomonas aeruginosa using translational fusions to MalE [J].

Epp, SF ;

Pechère, JC ;

Kok, M .

JOURNAL OF MICROBIOLOGICAL METHODS, 2001, 46 (01) :1-8

[8] C-terminal region of Pseudomonas aeruginosa outer membrane porin OprD modulates susceptibility to meropenem [J].

Epp, SF ;

Köhler, T ;

Plesiat, P ;

Michea-Hamzehpour, M ;

Frey, J ;

Pechère, JC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (06) :1780-1787

[9] Establishing clonal relationships between VIM-1-like metallo-β-lactamase-producing Pseudomonas aeruginosa strains from four European countries by multilocus sequence typing [J].

Giske, Christian G. ;

Libisch, Balazs ;

Colinon, Celine ;

Scoulica, Effie ;

Pagani, Laura ;

Fuzi, Miklos ;

Kronvall, Goran ;

Rossolini, Gian Maria .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (12) :4309-4315

[10] DISCRIMINATORY POWER OF 3 DNA-BASED TYPING TECHNIQUES FOR PSEUDOMONAS-AERUGINOSA [J].

GRUNDMANN, H ;

SCHNEIDER, C ;

HARTUNG, D ;

DASCHNER, FD ;

PITT, TL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :528-534

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