Complete nucleotide sequence of a maize chlorotic mottle virus isolate from Nebraska

被引：20

作者：

Stenger, Drake C. ^{[1
]}

French, Roy ^{[1
]}

机构：

[1] Univ Nebraska, USDA ARS, Dept Plant Pathol, Lincoln, NE 68583 USA

来源：

ARCHIVES OF VIROLOGY | 2008年 / 153卷 / 05期

关键词：

D O I：

10.1007/s00705-008-0069-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete genome of a maize chlorotic mottle virus isolate from Nebraska (MCMV-NE) was cloned and sequenced. The MCMV-NE genome consists of 4,436 nucleotides and shares 99.5 nucleotide sequence identity with an MCMV isolate from Kansas (MCMV-KS). Of 22 polymorphic sites, most resulted from transition with a clear bias for U to C and C to U substitutions. The MCMV-NE genome was assembled into a single plasmid insert and used as a template to transcribe RNA in vitro. As RNA transcribed from the cloned MCMV-NE genome was infectious to maize plants, sequence differences between MCMV-NE and MCMV-KS are most likely neutral with respect to pathogenicity and virulence.

引用

页码：995 / 997

页数：3

共 9 条

[1] Fully biologically active in vitro transcripts of the eriophyid mite-transmitted wheat streak mosaic tritimovirus [J].

Choi, IR ;

French, R ;

Hein, GL ;

Stenger, DC .

PHYTOPATHOLOGY, 1999, 89 (12) :1182-1185

[2] CONCENTRATION OF MAIZE CHLOROTIC MOTTLE VIRUS INCREASED IN MIXED INFECTIONS WITH MAIZE-DWARF MOSAIC-VIRUS, STRAIN-B [J].

GOLDBERG, KB ;

BRAKKE, MK .

PHYTOPATHOLOGY, 1987, 77 (02) :162-167

[3] CHARACTERIZATION OF MAIZE CHLOROTIC MOTTLE VIRUS [J].

LOMMEL, SA ;

KENDALL, TL ;

SIU, NF ;

NUTTER, RC .

PHYTOPATHOLOGY, 1991, 81 (08) :819-823

[4]

NIBLETT CL, 1978, PLANT DIS REP, V62, P15

[5] THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE MAIZE CHLOROTIC MOTTLE VIRUS GENOME [J].

NUTTER, RC ;

SCHEETS, K ;

PANGANIBAN, LC ;

LOMMEL, SA .

NUCLEIC ACIDS RESEARCH, 1989, 17 (08) :3163-3177

[6] TRANSCRIPTS OF A MAIZE CHLOROTIC MOTTLE VIRUS CDNA CLONE REPLICATE IN MAIZE PROTOPLASTS AND INFECT MAIZE PLANTS [J].

SCHEETS, K ;

KHOSRAVIFAR, R ;

NUTTER, RC .

VIROLOGY, 1993, 193 (02) :1006-1009

[7] Maize chlorotic mottle machlomovirus and wheat streak mosaic rymovirus concentrations increase in the synergistic disease corn lethal necrosis [J].

Scheets, K .

VIROLOGY, 1998, 242 (01) :28-38

[8] Wheat streak mosaic virus lacking helper component-proteinase is competent to produce disease Synergism in double infections with Maize chlorotic mottle virus [J].

Stenger, Drake C. ;

Young, Brock A. ;

Qu, Feng ;

Morris, T. Jack ;

French, Roy .

PHYTOPATHOLOGY, 2007, 97 (10) :1213-1221

[9] BIOLOGY AND CONTROL OF MAIZE CHLOROTIC MOTTLE VIRUS [J].

UYEMOTO, JK .

PLANT DISEASE, 1983, 67 (01) :7-10

← 1 →