Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes

被引:13
作者
Liu, SY
Sela, S
Cohen, G
Jadoun, J
Cheung, A
Ofek, I
机构
[1] TEL AVIV UNIV, SACKLER FAC MED, DEPT HUMAN MICROBIOL, IL-69978 TEL AVIV, ISRAEL
[2] TEL AVIV UNIV, FAC LIFE SCI, DEPT MOL MICROBIOL & BIOTECHNOL, IL-69978 TEL AVIV, ISRAEL
[3] ROCKEFELLER UNIV, LAB BACTERIAL PATHOGENESIS & IMMUNOL, NEW YORK, NY 10021 USA
关键词
Streptococcus pyogenes; streptolysin S; riboflavin; Tn916; mutagenesis;
D O I
10.1006/mpat.1996.0107
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Transposon Tn916 mutagenesis was used to create a mutant of Streptococcus pyogenes M type 3, designated ISS417, in which the ability to produce streptolysin S (SLS) and several other exoproteins was impaired. Concomitantly, the mutant became dependent upon riboflavin for growth and was able to grow in Todd Hewitt broth (THB) when supplemented with riboflavin or riboflavin-rich yeast extract. The parent strain was apparently able to utilize THB-derived components as a substitute for riboflavin, while the mutant was not. Although the parent strain grew well in synthetic medium, it was unable to produce SLS, except when it was supplemented with a small amount of THB. Thus, a component of THB was able to ''trigger'' SLS formation in the parent strain. The mutant grew well in this medium, but was unable to produce SLS even when it was supplemented with THB. Southern hybridization analysis revealed that ther ISS417 mutant harbours a single transposon insertion in its chromosome. Phage transduction experiments showed that the riboflavin dependency and the inability to make SLS phenotypes are co-transducible. The pleotrophic properties of the ISS417 mutant differ from those reported for insertional inactivation of the mga locus which regulates production of a number of surface proteins in S. pyogenes and the sar locus which regulates production of a number of exoproteins in Staphylococcus aureus. In view of the possibility that there exist a genetic linkage between the riboflavin biosynthetic pathway and expression of the oxygen-stable SLS, we hypothesize that SLS has a role in the growth economy of S. pyogenes. (C) 1997 Academic Press Limited.
引用
收藏
页码:227 / 234
页数:8
相关论文
共 38 条
[1]   PURIFICATION AND CHARACTERIZATION OF A PEPTIDE ESSENTIAL FOR FORMATION OF STREPTOLYSIN-S BY STREPTOCOCCUS-PYOGENES [J].
AKAO, T ;
TAKAHASHI, T ;
KOBASHI, K .
INFECTION AND IMMUNITY, 1992, 60 (11) :4777-4780
[2]   STREPTOCOCCAL TOXINS (STREPTOLYSIN-O, STREPTOLYSIN-S, ERYTHROGENIC TOXIN) [J].
ALOUF, JE .
PHARMACOLOGY & THERAPEUTICS, 1980, 11 (03) :661-717
[3]   ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR QUANTITATION OF ATTACHMENT AND INGESTION STAGES OF BACTERIAL PHAGOCYTOSIS [J].
ATHAMNA, A ;
OFEK, I .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (01) :62-66
[4]  
BERNHEIMER AW, 1972, STREPTOCOCCI STREPTO, P19
[5]  
Breese BB, 1978, BETA HEMOLYTIC STREP
[7]  
CAPARON MG, 1991, METHOD ENZYMOL, V204, P556
[8]   REGULATION OF EXOPROTEIN EXPRESSION IN STAPHYLOCOCCUS-AUREUS BY A LOCUS (SAR) DISTINCT FROM AGR [J].
CHEUNG, AL ;
KOOMEY, JM ;
BUTLER, CA ;
PROJAN, SJ ;
FISCHETTI, VA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6462-6466
[9]  
CLEARY PP, 1991, GENETICS AND MOLECULAR BIOLOGY OF STREPTOCOCCI, LACTOCOCCI, AND ENTEROCOCCI, P147
[10]   CLONING, SEQUENCING, AND EXPRESSION OF A FIBRONECTIN/FIBRINOGEN-BINDING PROTEIN FROM GROUP-A STREPTOCOCCI [J].
COURTNEY, HS ;
LI, Y ;
DALE, JB ;
HASTY, DL .
INFECTION AND IMMUNITY, 1994, 62 (09) :3937-3946