Determination of selenium in infant formulas whey fractions by SEC-HPLC-HG-ETAAS

被引：13

作者：

Bermejo, P

Barciela, J

Peña, EM

Bermejo, A

Fraga, JM

Cocho, JA

机构：

[1] Univ Santiago de Compostela, Fac Chem, Dept Analyt Chem Nutr & Bromatol, Santiago De Compostela 15706, Spain

[2] Univ Santiago de Compostela, Hosp Clin Univ, Lab Metab Disorders, Santiago De Compostela 15706, Spain

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2001年 / 16卷 / 02期

关键词：

D O I：

10.1039/b007065n

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method to determine the selenium distribution in milk whey fractions was developed. After milk whey preconcentration by ultrafiltration, selenium in the protein fractions was separated by size exclusion chromatography (SEC) and determined by electrothermal atomic absorption spectroscopy (ETAAS) using a sampling procedure based on fraction collection, fraction preconcentration by ultrafiltration and introduction into the furnace after digestion, hydride generation and hydride preconcentration. The digestion and reduction conditions of the milk whey fractions obtained by SEC were studied. The hydride generation parameters and preconcentration into an iridium coated graphite tube using ETAAS with a Zeeman background correction were optimised. The method was applied to six infant formula milks and selenium was found in three fractions of cow-based infant formulas: 40 kDa, > 160 kDa and mostly in the < 10 kDa fraction.

引用

页码：188 / 193

页数：6

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