Efficient regeneration from cotyledon protoplasts in Arabidopsis thaliana

被引：51

作者：

Dovzhenko, A ^{[1
]}

Dal Bosco, C ^{[1
]}

Meurer, J ^{[1
]}

Koop, HU ^{[1
]}

机构：

[1] Univ Munich, Dept Biol 1, Bereich Bot, Munich, Germany

来源：

PROTOPLASMA | 2003年 / 222卷 / 1-2期

关键词：

Arabidopsis thaliana; cotyledon; protoplast culture; shoot regeneration;

D O I：

10.1007/s00709-003-0011-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An efficient and fast regeneration system from cotyledon protoplasts was established for Arabidopsis thaliana accessions C24, Columbia, and Wassilewskija. Culture conditions and media compositions were optimised for the development of protoplasts embedded in thin alginate layers. Unexpectedly, the absence of cytokinins had a positive effect on cell development. Moreover, combined adjustment of alpha-naphthylacetic acid and dicamba concentrations resulted in high plating efficiencies of up to 30%, followed by shoot regeneration within only 19 days after protoplast isolation. The protocol is reproducible, efficient, extremely fast, and regenerated plants are fertile. Thus, this cotyledon-based system could prove useful for studying plant cell and molecular biology in A. thaliana.

引用

页码：107 / 111

页数：5

共 20 条

[1] REGENERATION OF FERTILE PLANTS FROM PROTOPLASTS OF DIFFERENT ARABIDOPSIS-THALIANA GENOTYPES [J].

DAMM, B ;

WILLMITZER, L .

MOLECULAR & GENERAL GENETICS, 1988, 213 (01) :15-20

[2] Thin-alginate-layer technique for protoplast culture of tobacco leaf protoplasts: shoot formation in less than two weeks [J].

Dovzhenko, A ;

Bergen, U ;

Koop, HU .

PROTOPLASMA, 1998, 204 (1-2) :114-118

[3] PLANT-REGENERATION FROM ARABIDOPSIS-THALIANA PROTOPLASTS [J].

FORD, KG .

PLANT CELL REPORTS, 1990, 8 (09) :534-537

[4] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[5] STABLE PLASTID TRANSFORMATION IN PEG-TREATED PROTOPLASTS OF NICOTIANA-TABACUM [J].

GOLDS, T ;

MALIGA, P ;

KOOP, HU .

BIO-TECHNOLOGY, 1993, 11 (01) :95-97

[6] Sugar beet guard cell protoplasts demonstrate a remarkable capacity for cell division enabling applications in stomatal physiology and molecular breeding [J].

Hall, RD ;

RiksenBruinsma, T ;

Weyens, G ;

Lefebvre, M ;

Dunwell, JM ;

VanTunen, A ;

Krens, FA .

JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 (307) :255-263

[7] Efficient plastid transformation in tobacco using the aphA-6 gene and kanamycin selection [J].

Huang, FC ;

Klaus, SMJ ;

Herz, S ;

Zou, Z ;

Koop, HU ;

Golds, TJ .

MOLECULAR GENETICS AND GENOMICS, 2002, 268 (01) :19-27

[8] Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].

Kaul, S ;

Koo, HL ;

Jenkins, J ;

Rizzo, M ;

Rooney, T ;

Tallon, LJ ;

Feldblyum, T ;

Nierman, W ;

Benito, MI ;

Lin, XY ;

Town, CD ;

Venter, JC ;

Fraser, CM ;

Tabata, S ;

Nakamura, Y ;

Kaneko, T ;

Sato, S ;

Asamizu, E ;

Kato, T ;

Kotani, H ;

Sasamoto, S ;

Ecker, JR ;

Theologis, A ;

Federspiel, NA ;

Palm, CJ ;

Osborne, BI ;

Shinn, P ;

Conway, AB ;

Vysotskaia, VS ;

Dewar, K ;

Conn, L ;

Lenz, CA ;

Kim, CJ ;

Hansen, NF ;

Liu, SX ;

Buehler, E ;

Altafi, H ;

Sakano, H ;

Dunn, P ;

Lam, B ;

Pham, PK ;

Chao, Q ;

Nguyen, M ;

Yu, GX ;

Chen, HM ;

Southwick, A ;

Lee, JM ;

Miranda, M ;

Toriumi, MJ ;

Davis, RW .

NATURE, 2000, 408 (6814) :796-815

[9] Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri) [J].

Kuchuk, N ;

Herrmann, RG ;

Koop, HU .

PLANT CELL REPORTS, 1998, 17 (08) :601-604

[10]

LUO Y, 1997, THESIS LUDWIG MAXIMI

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