An overview of the biophysical applications of atomic force microscopy

被引:153
作者
Santos, NC
Castanho, MARB
机构
[1] Fac Med Lisbon, Inst Mol Med, Inst Bioquim, P-1649028 Lisbon, Portugal
[2] Univ Lisbon, Fac Ciencias, Dept Quim & Bioquim, P-1749016 Lisbon, Portugal
[3] Inst Super Tecn, Ctr Quim Fis Mol, P-1049001 Lisbon, Portugal
关键词
atomic force microscopy; nucleic acid; supported membrane; protein; polysaccharide; non-topographical application;
D O I
10.1016/j.bpc.2003.09.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The potentialities of the atomic force microscopy (AFM) make it a tool of undeniable value for the study of biologically relevant samples. AFM is progressively becoming a usual benchtop technique. In average, more than one paper is published every day on AFM biological applications. This figure overcomes materials science applications, showing that 17 years after its invention, AFM has completely crossed the limits of its traditional areas of application. Its potential to image the structure of biomolecules or bio-surfaces with molecular or even sub-molecular resolution, study samples under physiological conditions (which allows to follow in situ the real time dynamics of some biological events), measure local chemical, physical and mechanical properties of a sample and manipulate single molecules should be emphasized. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:133 / 149
页数:17
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