Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

被引：20

作者：

Kaneko, YS

Mori, K

Nakashima, A

Nagatsu, I

Nagatsu, T

Ota, A ^{[1
]}

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Physiol, Toyoake, Aichi 4701192, Japan

[2] Fujita Hlth Univ, Sch Med, Dept Anat, Toyoake, Aichi 4701192, Japan

[3] Fujita Hlth Univ, Inst Comprehens Med Sci, Toyoake, Aichi 4701192, Japan

来源：

BRAIN RESEARCH PROTOCOLS | 2001年 / 8卷 / 01期

关键词：

locus coeruleus; tetrahydrobiopterin; GTP cyclohydrolase I; high-performance liquid chromatography (HPLC); differential oxidation; fluorescence detection;

D O I：

10.1016/S1385-299X(01)00081-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus. (C) 2001 Elsevier Science B V All rights reserved.

引用

页码：25 / 31

页数：7

共 24 条

[1]

BRAUTIGAM M, 1982, H-S Z PHYSIOL CHEM, V363, P341

[2] TETRAHYDROBIOPTERIN BIOSYNTHESIS - STUDIES WITH SPECIFICALLY LABELED (H-2)NAD(P)H AND (H2O)-H-2 AND OF THE ENZYMES INVOLVED [J].