Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF

被引:496
作者
Tsutsumi, S
Shimazu, A
Miyazaki, K
Pan, H
Koike, C
Yoshida, E
Takagishi, K
Kato, Y [1 ]
机构
[1] Japan Sci & Technol Corp, JST, Chiyoda Ku, Tokyo 1028666, Japan
[2] Hiroshima Univ, Fac Dent, Dept Biochem, Hiroshima 7348553, Japan
[3] Hiroshima Univ, Fac Dent, Dept Prevent Dent, Hiroshima 7348553, Japan
[4] Gunma Univ, Sch Med, Dept Orthopaed Surg, Maebashi, Gumma 3718511, Japan
关键词
mesenchymal stem cell; FGF; multilineage differentiation potential; life span;
D O I
10.1006/bbrc.2001.5777
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC. (C) 2001 Academic Press.
引用
收藏
页码:413 / 419
页数:7
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