Genetic transformation of Pseudomonas oleovorans by electroporation

被引：14

作者：

Solaiman, DKY ^{[1
]}

机构：

[1] ARS, USDA, NAA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 11期

关键词：

Pseudomonas oleovorans; electroporation; transformation; poly(beta-hydroxyalkanoate); alkane;

D O I：

10.1023/A:1008881121726

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electroporation procedure for the transformation of Pseudomonas oleovorans was developed using a model plasmid, pCN51. The optimal electrotransformation was achieved with cells harvested at 45 to 60 min of growth and concentrated to a cell density of 5 OD600nm, plasmid concentration of 6 mu g per 100 mu l of cell suspension, and a 0.1-cm gap-width cuvette. Electroporation was performed at the settings of 250 Omega, 2.5 mu F and 2.5 kV. Transformation yields in the order of 10(3) colony-forming-unit per electroporation sample were obtained. This is a first report of the electroporation of the commercially valuable bacterium Ps. oleovorans.

引用

页码：829 / 832

页数：4

共 15 条

[1] OXIDATION OF 1-ALKENES TO 1,2-EPOXYALKANES BY PSEUDOMONAS-OLEOVORANS [J].

ABBOTT, BJ ;

HOU, CT .

APPLIED MICROBIOLOGY, 1973, 26 (01) :86-91

[2] Synthesis of enantiopure epoxides through biocatalytic approaches [J].

Archelas, A ;

Furstoss, R .

ANNUAL REVIEW OF MICROBIOLOGY, 1997, 51 :491-525

[3] CHEMICAL AND BIOLOGICAL SYNTHESIS OF CHIRAL EPOXIDES [J].

BESSE, P ;

VESCHAMBRE, H .

TETRAHEDRON, 1994, 50 (30) :8885-8927

[4] GENETIC REGULATION OF OCTANE DISSIMILATION PLASMID IN PSEUDOMONAS [J].

CHAKRABARTY, AM ;

CHOU, G ;

GUNSALUS, IC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (04) :1137-1140

[5] The microbial production of poly(hydroxyalkanoates) from tallow [J].

Cromwick, AM ;

Foglia, T ;

Lenz, RW .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (5-6) :464-469

[6] CHARACTERIZATION OF INTRACELLULAR INCLUSIONS FORMED BY PSEUDOMONAS-OLEOVORANS DURING GROWTH ON OCTANE [J].

DESMET, MJ ;

EGGINK, G ;

WITHOLT, B ;

KINGMA, J ;

WYNBERG, H .

JOURNAL OF BACTERIOLOGY, 1983, 154 (02) :870-878

[7] BIOSYNTHESIS AND CHARACTERIZATION OF POLY(BETA-HYDROXYALKANOATES) PRODUCED BY PSEUDOMONAS-OLEOVORANS [J].

GROSS, RA ;

DEMELLO, C ;

LENZ, RW ;

BRANDL, H ;

FULLER, RC .

MACROMOLECULES, 1989, 22 (03) :1106-1115

[8] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[9] TRANSFORMATION OF PSEUDOMONAS-PUTIDA BY ELECTROPORATION [J].

IWASAKI, K ;

UCHIYAMA, H ;

YAGI, O ;

KURABAYASHI, T ;

ISHIZUKA, K ;

TAKAMURA, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (05) :851-854

[10] TRICHLOROETHYLENE AND CHLOROFORM DEGRADATION BY A RECOMBINANT PSEUDOMONAD EXPRESSING SOLUBLE METHANE MONOOXYGENASE FROM METHYLOSINUS-TRICHOSPORIUM OB3B [J].

JAHNG, DJ ;

WOOD, TK .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2473-2482

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