Studies of the Escherichia coli Rsd-σ70 complex

被引：32

作者：

Westblade, LF ^{[1
]}

Ilag, LL

Powell, AK

Kolb, A

Robinson, CV

Busby, SJW

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[2] Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England

[3] Inst Pasteur, Dept Microbiol Fondamentale & Med, F-75724 Paris, France

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2004年 / 335卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

Escherichia coli; transcription initiation; sigma-factors; anti-sigma factors; stoichiometry;

D O I：

10.1016/j.jmb.2003.11.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：685 / 692

页数：8

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