Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

被引：5

作者：

Dosio, F

Arpicco, S

Canevari, S

Figini, M

Gastaldi, D

机构：

[1] Univ Turin, Dipartimento Sci & Tecnol Farm, I-10125 Turin, Italy

[2] Ist Nazl Studio & Cura Tumori, Dipartimento Oncol Sperimentale, I-20133 Milan, Italy

[3] Univ Turin, Dipartimento Chim Analit, I-10125 Turin, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 830卷 / 02期

关键词：

membranes; immunotoxins; toxins; clavin; proteins; monoclonal antibodies;

D O I：

10.1016/S0021-9673(98)00933-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures. (C) 1999 Elsevier Science B.V. All rights reserved.

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收藏

页码：329 / 335

页数：7

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