Continuous two stage acetone-butanol-ethanol fermentation with integrated solvent removal using Clostridium acetobutylicum B 5313

被引：91

作者：

Bankar, Sandip B. ^{[1
,2
]}

Survase, Shrikant A. ^{[1
]}

Singhal, Rekha S. ^{[2
]}

Granstrom, Tom ^{[1
]}

机构：

[1] Aalto Univ, Sch Chem Technol, Dept Biotechnol & Chem Technol, Aalto 00076, Finland

[2] Inst Chem Technol, Dept Food Engn & Technol, Bombay 400019, Maharashtra, India

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 106卷

关键词：

Butanol; Two stage chemostat; Oleyl alcohol; Sugar cane bagasse; Clostridium acetobutylicum; 1ST STAGE; BEIJERINCKII; ABE; ACID;

D O I：

10.1016/j.biortech.2011.12.005

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The objective of this study was to optimize continuous acetone-butanol-ethanol (ABE) fermentation using a two stage chemostat system integrated with liquid-liquid extraction of solvents produced in the first stage. This minimized end product inhibition by butanol and subsequently enhanced glucose utilization and solvent production in continuous cultures of Clostridium acetobutylicum B 5313. During continuous two-stage ABE fermentation, sugarcane bagasse was used as the cell holding material for the both stages and liquid-liquid extraction was performed using an oleyl alcohol and decanol mixture. An overall solvent production of 25.32 g/L (acetone 5.93 g/L, butanol 16.90 g/L and ethanol 2.48 g/L) was observed as compared to 15.98 g/L in the single stage chemostat with highest solvent productivity and solvent yield of 2.5 g/L h and of 0.35 g/g, respectively. Maximum glucose utilization (83.21%) at a dilution rate of 0.05 1/h was observed as compared to 54.38% in the single stage chemostat. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：110 / 116

页数：7

共 34 条

[1] EFFECT OF PH AND BUTYRATE CONCENTRATION ON THE PRODUCTION OF ACETONE AND BUTANOL BY CLOSTRIDIUM-ACETOBUTYLICUM GROWN IN CONTINUOUS CULTURE [J].

BAHL, H ;

ANDERSCH, W ;

BRAUN, K ;

GOTTSCHALK, G .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1982, 14 (01) :17-20

[2]

BARTON WE, 1992, APPL MICROBIOL BIOT, V36, P632

[3] FORMATION OF BACTERIAL AGGREGATES IN A CONTINUOUS-FLOW REACTOR - INFLUENCE OF CARRIER AVAILABILITY [J].

BEEFTINK, HH ;

VANDILLEN, M ;

VANDENHEUVEL, JC .

BIOTECHNOLOGY LETTERS, 1988, 10 (03) :171-176

[4] CONTINUOUS DIRECT SOLVENT-EXTRACTION OF BUTANOL IN A FERMENTING FLUIDIZED-BED BIOREACTOR WITH IMMOBILIZED CLOSTRIDIUM-ACETOBUTYLICUM [J].

DAVISON, BH ;

THOMPSON, JE .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 39 :415-426

[5] ENHANCEMENT OF BUTANOL FORMATION BY CLOSTRIDIUM-ACETOBUTYLICUM IN THE PRESENCE OF DECANOL-OLEYL ALCOHOL MIXED EXTRACTANTS [J].

EVANS, PJ ;

WANG, HY .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (07) :1662-1667

[6] Improving performance of a gas stripping-based recovery system to remove butanol from Clostridium beijerinckii fermentation [J].

Ezeji, TC ;

Karcher, PM ;

Qureshi, N ;

Blaschek, HP .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2005, 27 (03) :207-214

[7]

Ezeji TC, 2006, CHEM IND-SER, V112, P99

[8] Long-term continuous cultivation of Clostridium beijerinckii in a two-stage chemostat with on-line solvent removal [J].

Gapes, JR ;

Nimcevic, D ;

Friedl, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (09) :3210-3219

[9] ACID IN THE 1ST STAGE IS A DETERMINANT FACTOR FOR THE SOLVENT PRODUCTION IN THE 2-STAGE CONTINUOUS FERMENTATION OF CLOSTRIDIUM-ACETOBUTYLICUM [J].

GODIN, C ;

ENGASSER, JM .

BIOTECHNOLOGY LETTERS, 1989, 11 (12) :903-906

[10]

Green E.M., 2011, CURR OPIN BIOTECH, V22, P1, DOI DOI 10.1016/J.C0PBI0.2011.02.004

← 1 2 3 4 →