Isolating cells from non-sperm cellular mixtures using the PALM® microlaser micro dissection system

被引：15

作者：

Anoruo, Belinda

van Oorschot, Roland

Mitchell, John

Howells, David

机构：

[1] Victoria Police Forens Serv Dept, Macleod, Vic 3085, Australia

[2] La Trobe Univ, Dept Genet & Human Variat, Bundoora, Vic 3086, Australia

[3] Austin Hosp, Dept Med, Heidelberg, Vic 3084, Australia

来源：

FORENSIC SCIENCE INTERNATIONAL | 2007年 / 173卷 / 2-3期

基金：

澳大利亚研究理事会;

关键词：

PALM (R); mixtures; trace; DNA; laser microdissection; pressure catapulting (LMPC);

D O I：

10.1016/j.forsciint.2007.01.031

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Use of the Positioning Ablation Laser MicroBeam (R) (PALW (R)) microlaser system to isolate specific cellular components from somatic cellular mixtures (blood and saliva) prior to DNA extraction and typing is compared with routine DNA extraction and typing of the same mixture samples. Mixtures of blood and saliva at differing ratios generated complex DNA profiles that included allele peaks originating from each of the donors, or just those of the major contributor. Isolation of cells with the PALM (R) microlaser prior to DNA extraction allowed informative, single-source, DNA profiles to be generated from a known cell type/origin. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：93 / 96

页数：4

共 18 条

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