The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages

被引:16
作者
Alvarez-Martinez, MT
Machold, J
Weise, C
Schmidt-Eisenlohr, H
Baron, C
Rouot, B
机构
[1] Univ Montpellier 2, INSERM U431, F-34095 Montpellier 05, France
[2] Free Univ Berlin, Inst Chem Biochem, D-14195 Berlin, Germany
[3] Univ Munich, Inst Genet & Mikrobiol, D-80638 Munich, Germany
关键词
D O I
10.1128/JB.183.18.5343-5351.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages.
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页码:5343 / 5351
页数:9
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共 46 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   SUGAR-MEDIATED INDUCTION OF AGROBACTERIUM-TUMEFACIENS VIRULENCE GENES - STRUCTURAL SPECIFICITY AND ACTIVITIES OF MONOSACCHARIDES [J].
ANKENBAUER, RG ;
NESTER, EW .
JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6442-6446
[3]  
Ausubel S. F., 1989, CURRENT PROTOCOLS MO
[4]   Signaling in plant-microbe interactions [J].
Baker, B ;
Zambryski, P ;
Staskawicz, B ;
DineshKumar, SP .
SCIENCE, 1997, 276 (5313) :726-733
[5]   Biochemistry of type IV secretion [J].
Burns, DL .
CURRENT OPINION IN MICROBIOLOGY, 1999, 2 (01) :25-29
[6]   Xenognosin sensing in virulence:: is there a phenol receptor in Agrobacterium tumefaciens? [J].
Campbell, AM ;
Tok, JB ;
Zhang, J ;
Wang, Y ;
Stein, M ;
Lynn, DG ;
Binns, AN .
CHEMISTRY & BIOLOGY, 2000, 7 (01) :65-76
[7]   SUGARS INDUCE THE AGROBACTERIUM VIRULENCE GENES THROUGH A PERIPLASMIC BINDING-PROTEIN AND A TRANSMEMBRANE SIGNAL PROTEIN [J].
CANGELOSI, GA ;
ANKENBAUER, RG ;
NESTER, EW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6708-6712
[8]   LIVE BRUCELLA SPP FAIL TO INDUCE TUMOR-NECROSIS-FACTOR-ALPHA EXCRETION UPON INFECTION OF U937-DERIVED PHAGOCYTES [J].
CARON, E ;
PEYRARD, T ;
KOHLER, S ;
CABANE, S ;
LIAUTARD, JP ;
DORNAND, J .
INFECTION AND IMMUNITY, 1994, 62 (12) :5267-5274
[9]   CONSTRUCTION OF AGROBACTERIUM STRAINS BY ELECTROPORATION OF GENOMIC DNA AND ITS UTILITY IN ANALYSIS OF CHROMOSOMAL VIRULENCE MUTATIONS [J].
CHARLES, TC ;
DOTY, SL ;
NESTER, EW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (11) :4192-4194
[10]   Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole [J].
Comerci, DJ ;
Martínez-Lorenzo, MJ ;
Sieira, R ;
Gorvel, JP ;
Ugalde, RA .
CELLULAR MICROBIOLOGY, 2001, 3 (03) :159-168