Isolation and identification of low molecular weight antioxidant compounds from fermented "chorizo" sausages

被引:27
作者
Broncano, J. M. [1 ]
Otte, J. [2 ]
Petron, M. J. [1 ]
Parra, V. [1 ]
Timon, M. L. [1 ]
机构
[1] Univ Extremadura, Agr Engn Sch, Dept Anim Prod & Food Sci, Badajoz 06007, Spain
[2] Univ Copenhagen, Fac Life Sci, Dept Food Sci, DK-1958 Frederiksberg C, Denmark
关键词
DPPH radical; Bioactive peptide; Chorizo; HILIC-MS/MS; LC-MS/MS; RADICAL-SCAVENGING ACTIVITIES; FREE AMINO-ACIDS; ELECTROSPRAY-IONIZATION; L-CARNITINE; PEPTIDES; PROTEIN; PROTEOLYSIS; CREATINE; CARNOSINE; ANSERINE;
D O I
10.1016/j.meatsci.2011.09.015
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
This work is focused on the determination of low molecular weight compounds extracted from samples of fermented sausages. The antioxidant activity of fractions isolated from chorizo extracts was tested by their ability to quench free radicals by the DPPH-radical scavenging assay. Natural dipeptides and metabolites characteristic of meat were abundant in the fractions isolated by RP-HPLC from chorizo extracts. Due to extensive degradation during the ripening of chorizo, the extracts did not contain many peptides in a concentration that allowed identification. However, many free amino acids were identified by LC-MS/MS and HILIC-MS/MS. The fractions with the most hydrophilic compounds showed the highest antioxidant activity. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:494 / 501
页数:8
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