Molecular analysis of archived blood slides reveals an atypical human Trypanosoma infection

被引：55

作者：

Deborggraeve, Stijn ^{[1
,6
]}

Koffi, Mathurin ^{[2
]}

Jamonneau, Vincent ^{[2
]}

Bonsu, Frank A. ^{[3
]}

Queyson, Richard ^{[4
]}

Simarro, Pere P. ^{[5
]}

Herdewijn, Piet ^{[6
]}

Buscher, Philippe ^{[1
]}

机构：

[1] Inst Trop Med, Dept Parasitol, B-2000 Antwerp, Belgium

[2] CIRDES, Bobo Dioulasso 01, Burkina Faso

[3] Ghana Hlth Serv, Dis Control Unit, Accra, Ghana

[4] Effia Nkwanta Reg Hosp, Takoradi, Ghana

[5] WHO, Dept Control Neglected Dis, CH-1211 Geneva, Switzerland

[6] Katholieke Univ Leuven, Rega Inst Med Res, Med Chem Lab, B-3000 Louvain, Belgium

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 04期

关键词：

Trypanosoma brucei; infection; spontaneous cure; molecular identification; Ghana;

D O I：

10.1016/j.diagmicrobio.2008.03.006

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In 2003, a 10-month-old Ghanaian boy recovered from a Trypanosoma brucei infection, although the patient was not treated with antitrypanosomal drugs. Only T. brucei gambiense and T. brucei rhodesiense are able to infect humans, causing human African trypanosomiasis. The disease is considered 100% fatal if left untreated. The identity of the trypanosome was determined by DNA extraction from the archived stained blood slides followed by sequential application of polymerase chain reactions (PCRs) that are specific for the order, subgenus, species and subspecies, followed by genotyping with microsatellite PCR. Molecular analysis indicated that the parasites observed in the patient's blood in 2003 belong to the T brucei subspecies brucei, which is normally not infectious to humans. Next to the clinical message, this article provides technical information to extract successfully DNA from archived blood slides for subsequent molecular analysis and to identify a trypanosorne by taxon-specific PCRs and microsatellite genotyping. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：428 / 433

页数：6

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