Note:: Identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction analysis

被引：55

作者：

Giraffa, G ^{[1
]}

De Vecchi, P ^{[1
]}

Rossetti, L ^{[1
]}

机构：

[1] Ist Sperimentale Lattiero Caseario, Microbiol Lab, I-26900 Lodi, Italy

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 1998年 / 85卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1998.00606.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Amplification and restriction analysis of the 16S rRNA gene (PCR-ARDRA) was used to identify Lactobacillus delbrueckii dairy isolates and to assign them to its three subspecies (delbrueckii, lactis and bulgaricus). PCR-ARDRA allowed confirmation of predictions from the DNA database regarding the restriction mapping of the 16S sequence to be verified in practice. PCR-ARDRA was shown to be a reliable and rapid method for identifying Lact. delbrueckii isolates at the subspecies level and for differentiating this species from Lact. helveticus and Lact. acidophilus.

引用

页码：918 / 924

页数：7

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