Molecular microbial ecology of nitrification in an activated sludge process treating refinery wastewater

被引:31
作者
Ballinger, SJ [1 ]
Head, IM
Curtis, TP
Godley, AR
机构
[1] Newcastle Univ, Fossil Fuels & Environm Geochem Postgrad Inst, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[2] Newcastle Univ, Dept Civil Engn, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[3] Shell Res Ltd, Thornton Res Ctr, Chester CH1 3SH, Cheshire, England
关键词
ammonia-oxidizing bacteria; molecular microbial ecology; nitrification; ribosomal RNA;
D O I
10.1016/S0273-1223(98)00091-2
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The diversity of autotrophic ammonia-oxidizing bacteria (AOB) of the b-subdivision of the-class Proteobacteria was investigated in a laboratory-scale denitrification-nitrification bioreactor (DNB) treating a synthetic waste stream. 16S ribosomal RNA (rRNA) gene sequences were amplified from DNA extracted from the oxic DNB sludge. Comparative analysis of the rRNA sequences revealed considerable diversity among the AOB-like sequences. The majority of sequences recovered were related to Nitrosomonas spp. but a smaller number of Nitrosospira-like sequences were obtained. Since different AOB may have different kinetic properties the high diversity of AOB, even in a simple laboratory biotreater treating a simple waste stream, has important implications for the operation of nitrifying wastewater treatment processes. (C) 1998 IAWQ. Published by Elsevier Science Ltd.
引用
收藏
页码:105 / 108
页数:4
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